Development and validation of a novel HPLC-UV method for the determination of faradiol in creams including Marigold (Calendula officinalis L.) extract

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A straightforward, quick, and sensitive high-performance liquid chromatography (HPLC) technique was created to quantify the triterpene alcohol faradiol present in cosmetic creams that contain extract from marigold (Calendula officinalis L.). The mobile phase consisted of 95:5 methanol:water (2% o-phosphoric acid) with an isocratic elution and a flow rate of 1.3 mL/min. The stationary phase was a C18 (5 μm × 4.6 mm × 150 mm) column. Throughout the separation, a constant temperature of 35 ºC was maintained. Thirty μL was the injection volume. At 6.38±0.20 min, the faradiol peak was eluted. The International Conference on Harmanization (ICH) criteria for linearity, limit of detection, limit of quantitation, selectivity, sensitivity, robustness, accuracy, and precision were followed in the validation process. The limits of quantification and detection were 0.01 μg/mL and 0.003, in respective order. The arrangement of linear expression was between 0.01 and 30 μg/mL. For both the daily and hourly analysis, the relative standard deviation values were less than 1.20%. A variety of cosmetic goods were analysed using the suggested approach. The technique is anticipated to be appropriate for regular triterpene alcohol faradiol analysis, quality assurance, and standardisation of cosmetic cream products.