The effect of exogenous proline and salicylic acid application on proline and apoplastic protein in cold tolerance of pepper callus cultures

OpenAlex English

In this study, the effect of proline and salicylic acid treatment on the amount of proline and apoplastic protein in cold tolerance of pepper callus cultures was investigated. Callus tissue developing in magenta boxes was transferred to an MS medium containing SA (0.25 and 0.50 mM), proline (12 and 24 mM) and SA + proline and developed under the same photoperiodic settings at 4, 8, 16 and 24°C. The maximum increases in proline at the lowest temperature (4°C) and the highest temperature (24°C) were observed to be in 24 mM proline + 0.25 mM SA and 24 mM proline + 0.50 mM SA treatments, respectively. The maximum increases in apoplastic protein at 4, 16 and 24°C were observed to be in co-administration of proline and salicylic acid, whereas the maximum increase in apoplastic protein at 8°C was observed to be in 12 mM proline treatment. Findings indicated that proline and salicylic acid treatment led to the accumulation of proline and apoplastic protein which increased the cold tolerance of pepper callus. © 2013 Friends Science Publishers