DETERMINATION AND MOLECULAR CHARACTERIZATION OF WATERMELON MOSAIC VIRUS IN CUCURBIT PLANTS IN TEKIRDAG PROVINCE IN TURKIYE

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A study was carried out to identify Watermelon mosaic virus (WMV) in cucurbit production areas in Tekirdag province of Turkiye.Infected plants were tested using Real-Time PCR and Reverse Transcriptase-PCR with oligonucleotides specific for the virus CP gene.Reverse Phylogenetic analysis of partial nucleotide sequences of the CP gene of 12 WMV isolates; TR-Tn2 (Genbank Acc.No. MT448602), TR-Tn5 (Genbank Acc.No. MT448603), TR-Tn8 (Genbank Acc.No. MT448604), TR-Tn9 (Genbank Acc.No. MT448605), TR-Tn10 (Genbank Acc.No. MT448606), TR-Tn18 (Genbank Acc.No. MT448607), TR-Tu75 (Genbank Acc.No. MT448612) and TR-Tu80 (Genbank Acc.No. MT448613); were showed 99.7-95% similarity to WMV ITA00-G (EU660590) from Italy, France isolate from France (NC_006262), TURK91 from Turkiye (EU660579), C05-464 from France (JF273459), CHI02-481 from Chile (EU660582), WMV-Pk from Pakistan (AB218280) and placed in WMV Group 1. Analysis of the 348 nucleotide of TR-Tf50 (MT448610), TR-Ta63 (MT448608), TR-Ta64 (MT448609) and TR-Ts71 (MT448611) isolates showed 98.2-92.2%similarity to WMV Watermelon isolate (AB369278) from South Korea, Ch99/69 (EF127832) and WMV-CHN (DQ399708) isolates from China, WMV TA-om3 (MN854651) from South Korea and S96-6 (AB353119) isolate from Japan and placed in WMV Group 3.