Article detail · 2011
Multiple Locus Variable Number Tandem Repeat Analysis Genotyping of Human Brucella Isolates from Turkey
- Year
- 2011
- Type
- article
Data source split
- YÖKSİS YÖKSİS article record
- YÖKSİS venue Journal of Clinical Microbiology
- Catalog match (ISSN) Journal of Clinical Microbiology
- OpenAlex OpenAlex enrichment (abstract, citations, topics)
Abstract
OpenAlex · English
A multiple-locus variable-number tandem-repeat analysis (MLVA) was applied to investigate the epidemiological relationship and genetic diversity among 162 human Brucella isolates collected from all geographic regions of Turkey in an 8-year period (2001 to 2008). The isolates were genotyped by using an MLVA assay developed in Orsay, France (MLVA-16(Orsay)) including eight minisatellite (panel 1) and eight microsatellite (panel 2, subdivided into 2A and 2B) markers. Panels 1 and 2A distinguish 14 genotypes; two of these represented 85% of the strains. Panel 2B displayed a very high discriminatory power. Three loci from panel 2B had diversity index values higher than 0.74. MLVA-16(Orsay) yielded 105 genotypes; 73 were represented by a unique isolate, and 32 included two to eight isolates. The isolates from different patients within the same outbreak or from the same patient before first-line therapy and after relapse showed identical genotypes. A number of MLVA genotypes appeared to be partially restricted to some geographic areas and displayed no annual variation, possibly reflecting persistence of genotypes in certain areas for a time span of at least a decade. This study, representing the first molecular typing results of human Brucella isolates from Turkey, indicated that Turkish human Brucella melitensis isolates were most closely related to the neighboring countries' isolates included in the East Mediterranean group.
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Citations
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77 citations
OpenAlex cited_by_count (cache / database)
30 publications in the local catalog that cite this work (OpenAlex reference match; not the full global list).
- Comparison of multiplex real-time polymerase chain reaction with serological tests and culture for diagnosing human brucellosis. 2019
- Comparison of multiplex real-time polymerase chain reaction with serological tests and culture for diagnosing human brucellosis 2019
- Comparison of multiplex real-time polymerase chain reaction with serological tests and culture for diagnosing human brucellosis 2019
- Cross border Molecular Tracing of Brucellosis in Europe 2012
- Investigation of antibiotic susceptibilities of Brucella Strains isolated from various clinical samples in eastern Turkey 2021
- Evaluation of the genetic profiles of Brucella melitensis strain from Turkey using multilocus variable number tandem repeat analysis (MLVA) and multilocus sequence typing (MLST) techniques 2022
- Evaluation of the genetic profiles of Brucella melitensis strain from Turkey using multilocus variable number tandem repeat analysis (MLVA) and multilocus sequence typing (MLST) techniques 2022
- Investigation of antibiotic susceptibilities of Brucella Strains isolated from various clinical samples in eastern Turkey 2021
- Osteoarticular involvement of brucellosis in pediatric patients: clinical and laboratory characteristics 2020
- Unrecognized pediatric and adult family members of children with acute brucellosis 2017