Utility of near infrared spectrophotometry in mesenteric ischemia: An experimental study

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This study investigated the effectiveness of near infrared (NIR) spectrophotometry for measuring abdominal saturation in a rat model of induced mesenteric ischemia. This was a randomized, controlled, non-blinded interventional animal study. Twenty-eight male Sprague Dawley rats were randomly allocated into four groups. Following induction of anesthesia, the abdominal region was shaved and intra-abdominal access was achieved through a 4-cm midline incision and basal measurements were performed using an INVOS-5100c device. The rats were grouped on the basis of rSO 2 measurement times. Mesenteric artery ligation was not performed on the control group rats. From the rest of the mesenteric artery ligated rats, measurements were taken at 30 min, at 2 h and at 6 h. Measurements were repeated using NIR spectrophotometry probes after the abdomen was surgically closed. Following the measurements, the abdomen was re-opened and small intestine tissue specimens were taken for histopathological examination. Initial and final NIR spectrophotometry measurements were compared. No statistically significant difference was found in the group 1 between basal rSO 2 values and post-incision values ( p = 0.748). There was a significant variation between the initial and final measurements in groups 2, 3, and 4 ( p = 0.018). Although various radiological or biochemical markers are available for detecting mesenteric ischemia, the use of NIR spectrophotometry can be considered a good non-invasive alternative method for early diagnosis of acute mesenteric ischemia.