The cytotoxicity and antioxidant activity analysis of the isolated constituents and extracts from endemic Centaurea derderiifolia

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Fatma Buluta, Ibrahim Demirtasa* , Serkan Koldasa , Fatih Gula , Lutfi Behcetb, Tevfik Ozenc & Feyza Oke-Altuntasda Department of Chemistry, Faculty of Science, Çankırı Karatekin University, Çankırı, Turkey; b Department of Biology, Faculty of Arts and Sciences, Bingöl University, Bingöl, Turkey; c Department of Chemistry, Faculty of Arts and Sciences, Ondokuz Mayıs University, Samsun, Turkey; d Department of Biology, Faculty of Science, Gazi University, Ankara, TurkeySupplemental data for this article can be accessed at https://doi.org/10.1080/14786419.2019.1644507.CONTACT Ibrahim Demirtas ibdemirtas@gmail.comAbstractIt is aimed to investigate the cytotoxic effects of the various extracts from the leaf and seed of Centaurea derderiifolia on the growth human cervical adenocarcinoma (HeLa) cells by xCELLigence method and to isolate the cytotoxic constituents. The results showed that subfractions 2 and 3 exhibited remarkable inhibitory effect against HeLa (IC50 < 10 µg/mL). The chloroform extract of leaf displayed the highest cytotoxic effect on HeLa cells (IC50 < 50 µg/mL) and was therefore subjected to a bioassay-guided multistep separation procedure. The pure compounds were elucidated by spectroscopic and mass-spectrometric analyses, including 1 D-, 2 D-NMR. In addition to cytotoxic effects of the isolated constituents, their antioxidant activities were also studied. On the other hand, subfraction 4 exhibited the highest DPPH radical scavenging activity (IC50 = 0.76 ± 0.03 mg/mL). β-sitosterol-3-O-β-d-glucopyranoside was isolated for the first time from this plant and three compounds from the bioactive subfractions were identified.