Article detail · 2003
Transcriptional activation via sequential histone H2B ubiquitylation and deubiquitylation mediated by SAGA associated Ubp8
Journal
Genes & DevelopmentISSN 0890-9369
The ISSN points to another catalog journal; the name is from the YÖKSİS record.
- Year
- 2003
- Type
- preprint
Data source split
- YÖKSİS YÖKSİS article record
- YÖKSİS venue Genes & Development
- Catalog match (ISSN) Genes and Development
- OpenAlex OpenAlex enrichment (abstract, citations, topics)
Abstract
OpenAlex · English
Gene activation and repression regulated by acetylation and deacetylation represent a paradigm for the function of histone modifications. We provide evidence that, in contrast, histone H2B monoubiquitylation and its deubiquitylation are both involved in gene activation. Substitution of the H2B ubiquitylation site at Lys 123 (K123) lowered transcription of certain genes regulated by the acetylation complex SAGA. Gene-associated H2B ubiquitylation was transient, increasing early during activation, and then decreasing coincident with significant RNA accumulation. We show that Ubp8, a component of the SAGA acetylation complex, is required for SAGA-mediated deubiquitylation of histone H2B in vitro. Loss of Ubp8 in vivo increased both gene-associated and overall cellular levels of ubiquitylated H2B. Deletion of Ubp8 lowered transcription of SAGA-regulated genes, and the severity of this defect was exacerbated by codeletion of the Gcn5 acetyltransferase within SAGA. In addition, disruption of either ubiquitylation or Ubp8-mediated deubiquitylation of H2B resulted in altered levels of gene-associated H3 Lys 4 methylation and Lys 36 methylation, which have both been linked to transcription. These results suggest that the histone H2B ubiquitylation state is dynamic during transcription, and that the sequence of histone modifications helps to control transcription.
Topics
Citations
OpenAlex cited_by_count. Not a WoS or Scopus citation count; those sources have no separate column here.
707 citations
OpenAlex cited_by_count (cache / database)
4 publications in the local catalog that cite this work (OpenAlex reference match; not the full global list).
- Maintenance of Low Histone Ubiquitylation by Ubp10 Correlates with Telomere Proximal Sir2 Association and Gene Silencing 2005
- H2B Ubiquitin Protease Ubp8 and Sgf11 Constitute a Discrete Functional Module within the Saccharomyces cerevisiae SAGA Complex 2005
- Histone H2B Ubiquitylation and Deubiquitylation in Genomic Regulation 2004
- Histone Post-Translational Modifications Regulate Transcription and Silent Chromatin in Saccharomyces cerevisiae 2006