Chemical composition of endemic and endangered Gypsophila pilulifera produced by in vitro micropropagation method

English (OpenAlex)

This article contains the result of the first study on the chemical composition of six methanol extracts of different plant parts of the Gypsophila pilulifera , which were grown in vitro and transferred to field conditions according to their distribution location. The methanol extracts of the leaves, branches, and roots of the G . pilulifera were obtained using the maceration method. All methanol extracts were derivatized with trimethylsilyl (-TMS) for gas chromatography-mass spectrometry (GC-MS) analysis. Fatty acid derivatives were the dominant group in all the extracts. Palmitic acid , ( Z , Z )− 9,12-octadecadienoic acid, and stearic acid were common fatty acids in all extracts. Sterols were detected in all methanol extracts. The predominant sterol was (3β,22 E )-Ergosta-7,22-dien-3-ol in the analyzed extracts. The determination of sterol compounds contributes to greater knowledge about the G . pilulifera and brings more attention to this plant species. G . pilulifera is an endemic and critically endangered (CR) species. The plant has potential use in different areas, especially in the pharmaceutical industry. In the current study, the chemical content of the underground and aboveground parts of G . pilulifera produced under in vitro conditions from the seeds collected from two different locations in nature was investigated comparatively according to their locations for the first time.