Investigation of the Phytochemical Components and Antiepileptic Potential of Helichrysum pallasii (Sprengel) Ledeb. Through in vitro and in vivo Studies

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This study investigated the bioactive components and in vitro and in vivo antiepileptic properties of the Helichrysum pallasii (Sprengel) Ledeb. species, which has long been used in traditional medicine in Eastern Anatolia. Pronounced antiepileptic properties of this plant were detected through in vitro antioxidant (ethanol and infusion=FCR: 112.0 and 60.0 mg GAE/gE; FRAP: 2687.5 and 1502.9 µmol Fe 2+ /gE; ORAC: 3200.7 and 2095.1 µmol Trolox Eq./gE; DPPH: IC50=440.5 and 661.0 µg ml-1; metal chelatin: IC50= 185.8 and 443.9 µg ml-1 respectively) and epilepsy-related enzyme Butyrylcholinesterase (IC50=374.5 and 817.9 µg ml-1 respectively), Acetylcholinesterase (IC50=242 and 682.0 µg ml-1 respectively) enzymes and GABA-T (IC50=350.3 and 676.4 µg ml-1 respectively) activity inhibition experiments and detailed in vivo investigations. In vivo, investigations included comparisons of the antiepileptic effects of three different dosages (60, 120, and 180 mg kg-1) of extract and infusion in a pentylenetetrazol kindling epilepsy model. It was found that the seizure scores were significantly reduced by both extracts, particularly at doses of 120 and 180 mg kg-1 (p