Simultaneous Determination of Acetaminophen Paracetamol and Ascorbic Acid in Pharmaceutical Formulations by LC Coupled to a Screen Printed Carbon Based Amperometric Detector

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Abstract The assay of acetaminophen (APAP) and ascorbic acid (AA) was performed by liquid chromatography (LC) with amperometric detection at a screen printed carbon based electrode poised at +0.6 V versus Ag pseudoreference electrode. A microparticle based conventional RP‐C18 and a monolithic RP‐C18 column were studied comparatively for the analytes separation using a mobile phase based on 0. 1 M phosphate buffer‐methanol 91 : 9 (v/v) at pH 6.5. The linear calibration range for both compounds was comprised within 2×10−7–1×10−4 M. Limit of detection (LOD) for APAP and AA was 2×10−7 and 5×10−7 M, respectively and the limit of quantification (LOQ) was 5. 10−7and 1×10−6 M, respectively. Square wave voltammetry at a Nafion modified glassy carbon electrode was developed and applied to the determination of APAP in order to validate the results obtained by the monolithic LC method for drug formulations.