Article detail · 2023
CRISPR/Cas-mediated plant genome editing: outstanding challenges a decade after implementation
- Year
- 2023
- Type
- article
Data source split
- YÖKSİS YÖKSİS article record
- YÖKSİS venue TRENDS IN PLANT SCIENCE
- Catalog match (ISSN) Trends in Plant Science
- OpenAlex OpenAlex enrichment (abstract, citations, topics)
Abstract
OpenAlex · English
The discovery of the CRISPR/Cas genome-editing system has revolutionized our understanding of the plant genome. CRISPR/Cas has been used for over a decade to modify plant genomes for the study of specific genes and biosynthetic pathways as well as to speed up breeding in many plant species, including both model and non-model crops. Although the CRISPR/Cas system is very efficient for genome editing, many bottlenecks and challenges slow down further improvement and applications. In this review we discuss the challenges that can occur during tissue culture, transformation, regeneration, and mutant detection. We also review the opportunities provided by new CRISPR platforms and specific applications related to gene regulation, abiotic and biotic stress response improvement, and de novo domestication of plants.
Topics
Citations
OpenAlex cited_by_count. Not a WoS or Scopus citation count; those sources have no separate column here.
215 citations
OpenAlex cited_by_count (cache / database)
12 publications in the local catalog that cite this work (OpenAlex reference match; not the full global list).
- Integrative approaches to enhance reproductive resilience of crops for climate-proof agriculture 2024
- Genome editing for healthy crops: traits, tools and impacts 2023
- Genome editing for healthy crops: traits, tools and impacts 2023
- Genome editing for healthy crops: traits, tools and impacts 2023
- CRISPR/Cas9-mediated mutagenesis of FT/TFL1 in petunia improves plant architecture and early flowering 2024
- Plant genome editing to achieve food and nutrient security 2024
- Establishing a reliable protoplast system for grapevine: isolation, transformation, and callus induction 2025
- The role of CRISPR in modern plant breeding: overcoming breeding barriers and legislative challenges through transgene-free genome editing 2026
- A rapid Agrobacterium rhizogenes-mediated transient expression for assessing sgRNA efficiency in CRISPR-Act3.0 in tomato 2026
- Challenges and Suggestions for Tissue Culture-Free Transformation and Genome Editing in De Novo Grapevine Meristems 2026