Antioxidant response of Chlamydomonas reinhardtii grown under different element regimes

OpenAlex · English

Summary Nutrient stress is one of the most favorable ways of increasing neutral lipid and high value‐added output production by microalgae. However, little is known about the level of the oxidative damage caused by nutrient stress for obtaining an optimal stress level for maximum production of specific molecules. In this study, the antioxidant response ofChlamydomonas reinhardtiigrown under element deprivation (nitrogen, sulfur, phosphorus and magnesium) and supplementation (nitrogen and zinc) was investigated. All element regimes caused a decrease in growth, which was most pronounced underNdeprivation. Element deprivation andZn supplementation caused significant increases in H2O2and lipid peroxidation levels ofC. reinhardtii. Decrease in total chlorophyll level was followed by an increase of total carotenoid levels inC. reinhardtiiunderNandSdeprivation while both increased underNsupplementation. Confocal imaging of live cells revealed dramatic changes of cell shape and production of neutral lipid bodies accompanied by a decrease of chlorophyll clusters. Antioxidant capacity of cells decreased underN,SandPdeprivation while it increased underNandZn supplementation. Fluctuation of antioxidant enzyme activities inC. reinhardtiigrown under different element regimes refers to different metabolic sources of reactive oxygen species production triggered by a specific element absence or overabundance.