Article detail · 2023
Cytotoxic effects of silver nanoparticles synthesized using Asphodelus aestivus Brot. aqueous extract
Journal
Journal of research in pharmacy (online)ISSN 2630-6344
The ISSN points to another catalog journal; the name is from the YÖKSİS record.
- Year
- 2023
- DOI
- 10.29228/jrp.413
- Type
- article
Data source split
- YÖKSİS YÖKSİS article record
- YÖKSİS venue Journal of research in pharmacy (online)
- Catalog match (ISSN) Journal of Research in Pharmacy
- OpenAlex OpenAlex enrichment (abstract, citations, topics)
Abstract
English (OpenAlex)
The aim of the study is to examine the cytotoxic, apoptotic effects and gene expressions of Asphodelus aestivus water extract (ASP) and silver nanoparticles (AgNPS) on decided cancer lines. Breast cancer cell lines MCF-7 and MDA-231; melanoma cancer lines MEWO and CHL-1; fibroblast cancer lines WI-38 and HEL 299 were selected for biological activities. xCELLigence system was used for cytotoxicity. Annexin V-EG FP Apoptosis detection kit was used for apoptosis and gene expressions were assessed by real time online RT-PCR by using cancer cell lines Qiagen kits. AgNPS showed significant cytotoxicity in all cell lines. The most prominent apoptosis was determined in MCF-7 cell line for AgNPS. It has been observed that the most important progress in gene expression, the suppression capacity of MUC1-C, a breast cancer-associated oncoprotein, is greatly increased by nanoparticle formation. In addition, cells that were not affected at all in MDA-MB-231 breast cancer oncoprotein started to suppress with nanoparticles. In conclusion, it was determined that silver nanoparticles increased the effect especially in breast cancer cell lines and could be considered for use.
Topics
- Phytochemistry and biological activity of medicinal plants
Primary topic Phytochemistry and biological activity of medicinal plants