Article detail · 2012
Amine intercalated montmorillonite matrices for enzyme immobilization and biosensing applications
- Year
- 2012
- Type
- article
Data source split
- YÖKSİS YÖKSİS article record
- YÖKSİS venue RSC Advances
- Catalog match (ISSN) RSC Advances
- OpenAlex OpenAlex enrichment (abstract, citations, topics)
Abstract
OpenAlex · English
Clay based biosensors were developed using montmorillonite (Mont) modified with methyl (M) and dimethylamine (DM). X-ray diffraction, Fourier transform infrared spectroscopy, zeta potential and thermal gravimetric measurements were used to characterize the modified clays. After immobilization of glucose oxidase (GOx) via clay on the glassy carbon electrode, its application as a glucose biosensor was investigated in detail. The best response characteristics were obtained by DM-Mont and optimization of enzyme amount, reproducibility of biosensor fabrication, repeatability of measurements and operational stability were all evaluated. The optimized biosensor showed a very good linearity between 0.05 mM and 1.0 mM, a 7 s response time and a limit of detection to glucose of 0.038 mM. Also, kinetic parameters and stabilities were determined. Apparent Km and Imax values were found as 0.73 mM and 2.955 μA, respectively. As well as batch configuration, the DM-Mont/GOx biosensor was successfully applied in the flow injection analysis mode. Finally, the performance of the DM-Mont/GOx biosensor to analyze glucose in a wine sample was compared with HPLC.
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Citations
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51 citations
OpenAlex cited_by_count (cache / database)
60 publications in the local catalog that cite this work (OpenAlex reference match; not the full global list).
- An electrospun nanofiber matrix based on organo-clay for biosensors: PVA/PAMAM-Montmorillonite 2018
- Molybdenum oxide/platinum modified glassy carbon electrode: A novel electrocatalytic platform for the monitoring of electrochemical reduction of oxygen and its biosensing applications 2013
- Molybdenum oxide platinum modified glassy carbon electrode A novel electrocatalytic platform for the monitoring of electrochemical reduction of oxygen and its biosensing applications 2013
- Molybdenum oxide platinum modified glassy carbon electrode A novel electrocatalytic platform for the monitoring of electrochemical reduction of oxygen and its biosensing applications 2013
- Molybdenum oxide platinum modified glassy carbon electrode A novel electrocatalytic platform for the monitoring of electrochemical reduction of oxygen and its biosensing applications 2013
- Molybdenum oxide platinum modified glassy carbon electrode A novel electrocatalytic platform for the monitoring of electrochemical reduction of oxygen and its biosensing applications 2013
- The effect of montmorillonite functionalization on the performance of glucose biosensors based on composite montmorillonite/PAN nanofibers 2020
- 4-aminothiophenol-intercalated montmorillonite: Organic-inorganic hybrid material as an immobilization support for biosensors 2020
- 4-aminothiophenol-intercalated montmorillonite: Organic-inorganic hybrid material as an immobilization support for biosensors 2020
- Enzyme immobilization in biosensor constructions: self-assembled monolayers of calixarenes containing thiols 2014