Prevalence of Methicillin Resistance and Panton-Valentine Leukocidin Genes in Staphylococci Isolated from Pirlak Sheep with Subclinical Mastitis in Turkey

OpenAlex İngilizce

This study aimed to investigate the presence of mecA and pvl genes in 47 Staphylococci previously isolated from 464 half-udder milk samples belong to 235 Pirlak sheep screening for subclinical mastitis. The species from Pirlak sheep used in the present study included: 13 S. aureus , 13 S. epidermidis , six S. xylosus , five S. chromogenes , three S. simulans , three S. hyicus , two S. warneri , one S. lentus and one S. saprophyticus . A total of 10 strains (21.3%) were determined to harbour mecA gene, of these, two (4.2%) also contained pvl gene. The strains carrying mecA gene were found to be S. aureus (3/13), S. xylosus (3/6), S. epidermidis (2/13), S. lentus (1/1) and S. hyicus (1/3). The presence of pvl gene was determined in a total of eight strains (17.0%), six (12.8%) of these were alone. Of pvl positive strains, three, three, one, and one were S. aureus , S. xylosus , S. simulans and S. hyicus , respectively. To our knowledge, this is the first study showing the presence of mecA and pvl genes in the Staphylococci isolated from Pirlak sheep with subclinical mastitis in Turkey.