Investigation of Methicillin and Panton-Valentine Leukocidin Genes in Staphylococcus aureus Strains Isolated from Clotted Creams Sold in Afyonkarahisar

OpenAlex İngilizce

This study aimed to investigate the methicillin and Panton-Valentine leukocidin genes in Staphylococcus aureus strains isolated from clotted cream samples produced and sold in Afyonkarahisar. A total of 110 clotted cream samples sold in public bazaars of Afyonkarahisar were collected between November 2019 and December 2020. Conventional cultural methods achieved the isolation of S. aureus from clotted cream samples. For the confirmation of S. aureus strains isolated from samples and determination of mecA and pvl genes in the strains, PCR was used. In this study, while S. aureus was isolated from 14 of 110 clotted cream samples by standard cultural methods, 13 (11.8%) of 110 samples were typed to be S. aureus by PCR. The mecA and pvl genes were found in none of the 13 S. aureus strains. In this study, in which the pvl gene was investigated for the first time in the S. aureus strains isolated from clotted creams in Turkey, it was thought that more research should be done to determine mecA and pvl genes in this traditional product and other dairy products.