Endo-pectinase Production by Bacillus pumilus NRRL B-212 and Optimization by RSM using Sugar Beet Pulp

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The present study aimed at optimization of culture conditions for endo-pectinase production by Bacillus pumilus NRRL B-212.In the first stage of the study, submerged fermentation experiments were performed to investigate effects of initial pH, carbon and nitrogen sources, salts and phosphate on endo-pectinase activity and maximum enzyme production was at pH: 8.The effect of initial pectin concentration on enzyme production was examined, and 1 % (w/v) pectin concentration was selected as the optimum pectin concentration.Yeast extract, (NH 4 ) 2 SO 4 , and peptone were used as nitrogen sources, and the medium containing 0.05 % (w/v) ammonium sulphate was the medium where maximum activity was achieved.In the experiments investigating the effect of salts, the maximum activity value was determined in the medium containing 0.02 % NaCl.In addition, the effect of phosphate concentration on enzyme production was investigated, and the highest endo-pectinase activity was determined in medium containing 0.3 % K 2 HPO 4 + 0.15 % KH 2 PO 4 .In the second stage of the study, solid-state fermentation studies were performed, and sugar beet pulp was used as agricultural waste.In order to obtain maximum endo-pectinase production and reveal the parameters influencing enzyme activity using sugar beet pulp, a Central Composite Design (CCD) was applied.The highest endo-pectinase activity was obtained as 147.75 U mL -1 in medium containing 6.78 % sugar beet pulp, 0.48 % (NH 4 ) 2 SO 4 and 0.12 % yeast extract.