Efficiency of P-Coumaric Acid and Ellagic Acid Added to Preservation Solutions in Transplantation

İngilizce (OpenAlex)

OBJECTIVE: In this study, it was aimed to evaluate the protective efficacy of the University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) preservation solutions enriched with P-coumaric acid and ellagic acid. MATERIAL AND METHOD Thirty-six Wistar albino male rats were divided into equal 6ng groups: (Group 1) UW; (Group 2) UW + P-coumaric; (Group 3) UW + Elagic; (Group 4) HTK ;. (Group 5) HTK + P-Coumaric; (Group 6) HTK + Elagic. After the liver perfusion was performed, samples were taken from the liver tissue and perfusion fluid at 0, 6 and 12 hours. At the sixth hour, liver biopsies were performed for histopathological and electron microscopic examination. RESULTS In groups enriched with p-coumaric acid (group 2 and group 5), antioxidant enzyme activities, which were higher than the control groups, were decreased at the 0th hour and the difference disappeared at the 6th and 12th hour. In the groups enriched with Ellagic acid, the highest antioxidant enzyme activity was in group 6, when the groups were evaluated in terms of cytokine activity, which are inflammatory parameters, the group with the highest anti-inflammatory cytokines IL4 and IL10 was found to be in group 6, and in group 3, the results were found with group 6. it was found to be similar. In group 2 and group 5, although the results were lower than the control group, the difference was not significant (P> 0.05). It was observed that the results in group 6, in which the lowest values ​​in terms of inflammatory cytokines TNF-α, IL1 and IL6 were in group 3, were higher than in group 3, but significantly lower than the control group, especially in terms of IL6 values ​​(P