Makale detayı · 2018
An evaluation for the standardization of the Alliumcepa test as cytotoxicity and genotoxicity assay
- Yıl
- 2018
- Tür
- article
Veri kaynağı ayrımı
- YÖKSİS YÖKSİS makale kaydı
- YÖKSİS dergi adı CARYOLOGIA
- Katalog eşleşmesi (ISSN) Caryologia
- OpenAlex OpenAlex zenginleştirmesi (özet, atıf, konular)
Özet
OpenAlex · İngilizce
A general report on the use of the Allium test as cytotoxicological and genotoxicological assay is proposed, with particular emphasis about the standardization of the test in several common applications. The intraspecific variation in Allium cepa has been overlooked, as in most investigations no mention is made about origin and denomination of the onion cultivar used. A standardization of the used material would allow a better generalization of the results, since we cannot be sure that all cultivars would give the same response. A more frequent use of transmission electron microscopy (TEM) investigation is proposed. Even if relatively time consuming and not available in all laboratories, it may help to better understand the mechanism of cytotoxicity, since many morphological characters may appear similar but be arisen from different processes observable only with TEM. About statistical testing, tests other than chi-squared may be used in case of a lower amount of data. The most commonly used statistical tests are the parametric tests ANOVA and Student’s t, and the non-parametric tests Kruskal–Wallis and Mann–Whitney U, for analysis of variance. Tests should be used also to assess the minimal sample dimension for obtaining significance, since data collection (microscope observation) appears to be one of the main bottle necks of the test. Also the use of the Allium test for testing liposomes and other nanovectors for drug delivery is proposed, in order to assess the cytotoxicity of these types of medium and the possible increase in cytotoxicity of the associated drug.
Konular
Atıflar
OpenAlex cited_by_count. WoS veya Scopus atıf sayısı değildir; o kaynaklar için ayrı kolon yoktur.
191 atıf
OpenAlex cited_by_count (önbellek / veritabanı)
Yerel katalogda bu makaleye atıf yapan 39 yayın (OpenAlex referans eşleşmesi; tam dünya listesi değildir).
- Genotoxic and cytotoxic effects of pethoxamid herbicide on Allium cepa cells and its molecular docking studies to unravel genotoxicity mechanism 2022
- Assessment of the Pharmacological Properties and Phytochemical Profile of Bruguiera gymnorhiza (L.) Lam Using In Vitro Studies, In Silico Docking, and Multivariate Analysis 2020
- Assessment of the ameliorative effect of curcumin on pendimethalin-induced genetic and biochemical toxicity 2022
- Acute multiple toxic efects of Trifoxystrobin fungicide on Allium cepa L. 2022
- Cyto-Genotoxic and Behavioral Effects of Flubendiamide in Allium cepa Root Cells, Drosophila melanogaster and Molecular Docking Studies 2023
- Cyto-Genotoxic and Behavioral Effects of Flubendiamide in Allium cepa Root Cells, Drosophila melanogaster and Molecular Docking Studies 2023
- Effect of Melissa officinalis L. leaf extract on manganese-induced cyto-genotoxicity on Allium cepa L. 2023
- Therapeutic effects of royal jelly against sodium benzoate-induced toxicity: cytotoxic, genotoxic, and biochemical assessment 2021
- Turmeric (Curcuma longa L.) tends to reduce the toxic effects of nickel (II) chloride in Allium cepa L. roots 2022
- Turmeric (Curcuma longa L.) tends to reduce the toxic effects of nickel (II) chloride in Allium cepa L. roots 2022