Evaluating Antibiofilm, Cytotoxic and Apoptotic Activities ofScutellaria brevibracteatasubsp.brevibracteataEssential Oil

OpenAlex İngilizce

Abstract Essential oils have many important biological properties, including antibacterial and antibiofilm activities. These unique properties make, essential oils good alternatives to synthetic chemical drugs, which have many side effects. In this study, we aimed to determine the chemical composition and biological activity of the essential oil obtained fromScutellaria brevibracteatasubsp.brevibracteata. Specifically, its antibiofilm activity againstPseudomonas aeruginosaPAO1 andStaphylococcus aureusATCC 29213 biofilms using XTT assay. Cytotoxic and apoptotic properties of the essential oil were investigated in human lung cancer cells (A540 and H1299) using MTT assay, Annexin V‐FITC and propidium iodide staining and q‐PCR. Thirty‐two different compounds were identified from the essential oil, of which elemol (20.42 %), γ‐eudesmol (20.12 %) and β‐eudesmol (14.85 %) were the main components. The essential oil was more effective againstP. aeruginosaPAO1 biofilm (79 %) thanS. aureusATCC 29213 biofilm (27 %). The specific activity of the essential oil againstP. aeruginosabiofilm may be related to its high terpene contents. In addition, the essential oil showed high cytotoxic activity towards A549 (IC509.09 μg/ml) and H1299 (IC5055.04 μg/ml) cell lines, inducing apoptosis in these cancer cells. These results demonstrate the antibiofilm and anticancer activities ofS. brevibracteatasubsp.brevibracteataessential oil.