Advanced Fluorescence Microscopy Techniques

According to quantum chromodynamics, at sufficiently high energy, the structure of hadrons reveals a dynamic equilibrium between gluon splitting and gluon recombination-a phenomenon known as saturation. The process of diffractive photonuclear production of a J/ψ vector meson provides a direct insight into the gluon co…

Reflectance confocal microscopy (RCM) provides noninvasive, cellular-resolution "optical biopsies" of human skin \emph{in vivo} by acquiring en-face images at successive depths, forming a sparse z-stack. Due to optical limitations, these stacks are anisotropic 3D volumes with lateral resolution (0.5 $μ$m) $\sim$6 time…

Reflectance confocal microscopy (RCM) provides noninvasive, cellular-resolution "optical biopsies" of human skin \emph{in vivo} by acquiring en-face images at successive depths, forming a sparse z-stack. Due to optical limitations, these stacks are anisotropic 3D volumes with lateral resolution (0.5 $μ$m) $\sim$6 time…

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Immunohistochemistry is a tissue-based technique that enables in situ visualization of specific molecules, most commonly proteins or peptides, within preserved cellular and tissue architecture through antigen-antibody specificity. By using labeled antibodies, immunohistochemistry allows spatially resolved and cell typ…

Abstract Laser-based microscopy systems require precise control of beam propagation, not only for high-quality image formation, but also for enabling quantitative measurements and detailed studies of laser–matter interactions. In this work, we investigate the propagation of non-paraxial Gaussian beams in an optical mi…

ABSTRACT Förster Resonance Energy Transfer (FRET) analysis is a powerful technique for studying protein–protein interactions; however, manual methods often introduce variability and user dependency. We present the SONLab FRET Analysis Tool, an open‐source and automated software that integrates Cellpose for cell segmen…

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ABSTRACT The two new N ‐tosylated carbazole derivatives (CTCl and CTMe) were synthesized and their binding properties with the two proteins (HSA and lysozyme) have been examined by UV absorption, fluorescence (steady‐state and 3D) spectroscopies and computational methods. Fluorescence measurements revealed that the de…

• ELMAS GÖKOĞLU

ABSTRACT The two new N ‐tosylated carbazole derivatives (CTCl and CTMe) were synthesized and their binding properties with the two proteins (HSA and lysozyme) have been examined by UV absorption, fluorescence (steady‐state and 3D) spectroscopies and computational methods. Fluorescence measurements revealed that the de…

• HALİL DUYAR

Immunohistochemistry is a tissue-based technique that enables in situ visualization of specific molecules, most commonly proteins or peptides, within preserved cellular and tissue architecture through antigen-antibody specificity. By using labeled antibodies, immunohistochemistry allows spatially resolved and cell typ…

• AZİZ AKSOY

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• MURAT AYDEMİR