Screening of Bioactive Potential of Plasteurhynchium striatulum: Antibiofilm Effects and Phytochemical Profile

OpenAlex İngilizce

In the present study, the bryophyte Plasteurhynchium striatulum (Spruce) M. Fleisch was extracted using three different solvents: ethanol, methanol, and n-hexane. Subsequently, the antimicrobial activities of the extracts were evaluated against a range of microorganisms including foodborne isolates, clinical isolates, multidrug-resistant strains, and standard strains using disk diffusion, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and antibiofilm assays. In addition, antioxidant activity was assessed through the DPPH radical scavenging assay. The biochemical constituents of the extracts were identified via gas chromatography–mass spectrometry (GC-MS) analysis. According to the disk diffusion test results, activity was observed against eight bacterial strains, most notably Escherichia coli and Providencia rustigianii, both of which exhibit multidrug resistance. The highest MIC value was recorded as 0.8816 mg/mL against the Salmonella infantis strain. The strongest antioxidant performance was observed at a concentration of 1 mg/mL. GC-MS analysis revealed that the major components of the extract were Tris(2,4-di-tert-butylphenyl) phosphate (52.45%), A'-Neogammacer-22(29)-ene (25.00%), and Neophytadiene (12.72%). In conclusion, P. striatulum was found to possess notable antimicrobial and antioxidant activities, along with a rich biochemical composition. These findings suggest that P. striatulum holds promise as a potential source for the development of novel antimicrobial and antioxidant agents. Additionally, its biochemical characteristics could offer important information for upcoming studies.