Investigation of the potential probiotic effects of lactic acid bacteria and cell-free supernatants against important pathogens leading to wound infections

İngilizce (OpenAlex)

BACKGROUND: In recent years, several in-vitro studies have revealed that some of the LAB strains and their cell-free supernatants (CFSs) have shown antibacterial activities and promising tools to prevent or disperse the biofilm formation of dermatological pathogens. In this study, it was aimed to investigate the antibacterial effects of LAB active cultures and CFSs against P. aeruginosa and S. aureus which were related to wound infections, as well as the inhibitory effects of CFSs on the biofilm formation of pathogens and destroying biofilms.METHODS: Active cultures were evaluated by agar spot and cell-free supernatants were evaluated by agar well diffusion method. Quantitative microdilution plate method was used for biofilm experiments. Antibiotic susceptibility profiles of isolates were evaluated by E-test method.RESULTS: The current results indicated that some of the LAB active cultures and CFSs have shown antibacterial properties against pathogens. Furthermore, it was determined that some of the CFSs have shown powerful inhibitory effects on biofilm formation and destroying biofilms.CONCLUSIONS: All data together signify a great ability of the applications of CFSs as antibacterial and biofilm removal compounds that could be very suitable to control wound infections caused by these pathogens.