Investigating the Effect of Different Growth Media on Biomass Production of Pseudopediastrum boryanum (Turpin) E. Hegewald Isolates

OpenAlex İngilizce

Microalgae Pseudopediastrum boryanum (Turpin) E. Hegewald was chosen as a subject for the present research due to its potential uses of wastewater treatment and biodiesel production. In the present study, we investigated the growth and biomass production of P. boryanum through use of semi-continuous cultures employing two growth media (Allen and BG-11). In our previous study, P. boryanum was isolated from different freshwater reservoir through the dilution technique. The isolated P. boryanum strain was inoculated with 270 mL of medium + 30 mL of suspension culture and the 16:8 light/dark photoperiod was applied. Optical density was recorded by using UV-Visible spectrophotometer at 670 nm, and cell count examination was performed through drop count method. Besides, dry weight and chlorophyll-a concentration of strain were determined. The highest cell density (3.67x106 cells/mL), dry weight (0.032 g/mL) and chlorophyll-a (16.39 µgL-1) production were observed in the Allen medium. Growth rates of P. boryanum were found to be 0.6676 d-1 in the Allen and 0.6021 d-1 in the BG-11 medium.