Recombinant adiponectin inhibits inflammation processes via NF-kB pathway in acute pancreatitis

OpenAlex İngilizce

PURPOSE: Adiponectin is a protein stemming from adipose tissue and having strong anti-inflammatory properties. We aimed to assess the damage diminishing effects of recombinant adiponectin (rAD) through NF-kB in the experimental acute pancreatitis (AP) model. MATERIALS AND METHODS: Acute pancreatitis was created by applying 50 µg/kg dose of intraperitoneal cerulean. The rats were randomised and divided into 3 groups as control, AP and rAD groups. Moreover, the rats in each group were divided into two sub-groups as 24th and 48th hour subgroups. rAD was injected in the study group intraperitoneally. Tissue and blood samples were taken after 24 and 48 hours. Histopathological assessment and NF-kB activity were investigated in pancreatic tissue. RESULTS: Serum TNF-a, IL-1b and IL-6 levels were found to be statistically significant in the AP group compared to the rAD group in the 24th and 48th hour (p < 0.05). Similarly, NF-kB activity was also found to be significant in the AP group both in the 24th and 48th hour (p < 0.05). There were significant differences in the AP and the rAD groups histopathologically in terms of edema, inflammation, vacuolisation and necrosis (p < 0.001). CONCLUSION: rAD has significantly reduced NF-kB activity, cytokine levels and tissue damage (Tab. 1, Fig. 1, Ref. 51).