Follicular fluid cerebellin and betatrophin regulate the metabolic functions of growing follicles in polycystic ovary syndrome

OpenAlex İngilizce

Objective: The aim of this study was to assess the changes of follicular fluid (FF) and serum levels of cerebellin precursor protein 1 (cbln1) and betatrophin in patients with polycystic ovary syndrome (PCOS) undergoing in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) with a gonadotropin-releasing hormone (GnRH) antagonist protocol.Methods: Twenty infertile women with PCOS and 20 control women diagnosed as poor responders undergoing ovarian stimulation with a GnRH antagonist were included.Blood samples were obtained during ovum pick-up.Follicular fluid from a dominant follicle was collected from the subjects.Using enzyme-linked immunosorbent assays, FF and serum levels of cbln1 and betatrophin were measured in both groups of participants.Metabolic and hormonal parameters were also determined and correlated with each other.Results: Both groups of women had similar serum and FF betatrophin levels (55.0 ± 8.9 ng/mL vs. 53.1 ± 10.3 ng/mL, p= 0.11).The serum and FF betatrophin levels of poor responders were found to be similar (49.9 ± 5.9 ng/mL vs. 48.9± 10.7 ng/mL, p= 0.22).Conversely, the FF cbln1 levels of PCOS women were found to be significantly higher than the serum cbln1 levels (589.1 ± 147.6 ng/L vs. 531.7 ± 74.3 ng/L, p< 0.02).The FF cbln1 levels of control participants without PCOS were significantly higher than their serum cbln1 levels (599.3 ± 211.5 ng/L vs. 525.3± 87.0 ng/L, p= 0.01).Positive correlations were detected among body mass index, insulin resistance, serum insulin, total testosterone, and betatrophin levels in the PCOS group.Conclusion: Follicular fluid betatrophin and cbln1 concentrations may play a pivotal role on follicular growth in PCOS subjects undergoing IVF/ ICSI with an antagonist protocol.