ISSR Marker Based Genetic Variation Analysis in the Riparian Relict Tree Pterocarya fraxinifolia (Juglandaceae) Genotypes in Türkiye

OpenAlex İngilizce

Abstract In this study, ISSR-PCR marker technique was used to determine the genetic variation of Pterocarya fraxinifolia genotypes collected from Düzce, Samsun, Siirt, Hatay, Kilis, Gaziantep, Kahramanmaraş and Adıyaman provinces. In this study, 71.95% polymorphism was obtained from 12 ISSR primers. A mean polymorphism information content (PIC) value of 0.3206 was found. The mean gene diversity (H) and major allele frequency values were found to be 0.4078 and 0.6873, respectively. The unweighted pair group method with arithmetic mean (UPGMA) dendrogram consists of two groups. In the dendrogram, the clustering of the genotypes proved to be geographically compatible. Genetic matrix distances between genotypes varied between 0.01220 and 0.44118. A total of 80% (eigen >1) of the genotype variation was explained by the first five eigenvalues and 19 genotypes were reduced to two dimensions. As a result, the ISSR marker technique has proved to be productive in determining the genetic variation and phylogenetic relationships of P. fraxinifolia genotypes.