Evaluation of Serum CTNNβ1 and E-Cadherin Levels inHepatitis Patients

OpenAlex İngilizce

Objectives: Hepatitis B virus (HBV) infection is one of the leading causes of hepatocellular carcinoma, but the underlying molecular mechanisms are quite complex.In this study, the aim was to reveal the relationship of these parameters with HBV-DNA loads by evaluating serum CTNNβ 1 (β-catenin) and E-cadherin levels in hepatitis B patients. Materials and Methods:In this study, between the dates of June 15.06.2018-December 30.12.2019, 75 people who were diagnosed with chronic hepatitis B constituted the patient group (n=75), and 75 people who were not diagnosed with chronic hepatitis B constituted the healthy control group (n=75).In this retrospective study using a random sampling method, the hepatitis B patient group was classified into 3 separate groups among themselves according to HBV-DNA loads; HBV-DNA-1 (x10 6 -10 8 , n=25), HBV-DNA-2 (x10 3 -10 4 , n=25) and HBV-DNA-3 (x10 1 -10 2 , n=25).While the level of serum β-catenin and E-cadherin, the main parameters in the study, were measured using the ELISA method with a commercial kit, the Chemiluminescent Microparticle Immunoassay method was used to evaluate the serological markers in the patients.HBV-DNA level was determined by real-time polymerase chain reaction.Results: In our study, the average age of individuals was 41.96±13.86 years in the control group and 36.72±16.3,42.8±10.91and 46.36 ±12.58 years in the HBV-DNA-1, 2, and 3 groups, respectively.The low age in the HBV-DNA-1 group compared to other groups was statistically significant (p=0.001;p<0.01).The values of E-cadherin Amaç: Hepatit B virüsü (HBV) enfeksiyonu, hepatosellüler karsinomun önde gelen nedenlerinden biridir, ancak altta yatan moleküler mekanizmalar ise oldukça karmaşıktır.Bu çalışmada hepatit B hastalarında serum CTNNβ 1 (β-katenin) ve E-kadherin düzeylerinin değerlendirerek bu parametrelerin HBV-DNA yükleriyle ilişkinin ortaya konması amaçlanmıştır.Gereç ve Yöntemler: Bu çalışmada; 15.06.2018-30.12.2019 tarihleri arasında kronik hepatit B tanısı alan 75 kişi hasta grubunu (n=75), kronik hepatit B tanısı almayan 75 kişi ise sağlıklı konrtol grubunu (n=75) oluşturdu.Rastgele örnekleme yöntemi kullanılan bu retrospektif çalışmada hepatit B hasta grubu HBV-DNA yüklerine göre kendi arasında; HBV-DNA-1 (x10 6 -10 8 , n=25), HBV-DNA-2 (x10 3 -10 4 , n=25) ve HBV-DNA-3 (x10 1 -10 2 , n=25) olmak üzere 3 ayrı gruba ayrıldı.Çalışmanın ana parametresi olan serum β-katenin ve E-kadherin düzeyi, ticari kit kullanılarak ELİSA yöntemi ile ölçülürken, hastaların serolojik belirteçlerin değerlendirilmesinde Kemilüminesan Mikropartikül İmmünoassay yöntemi kullanıldı.HBV-DNA düzeyi ise real-time polimeraz zincir reaksiyonuyla belirlendi.