Evaluation of serum interleukin-33 and gene polymorphisms in patients with bronchial asthma

OpenAlex İngilizce

Aims: Asthma is a chronic inflammatory disease of the airways.The aim of the study was to investigate the association between serum interleukin (IL)-33 level and IL-33 gene rs1342326, rs1929992 and rs3939286 polymorphisms in patients with asthma.Methods: Ninety patients aged 18 years and older, who were diagnosed with asthma, and 90 healthy control subjects were included in the study.Serum IL-33 levels were measured by enzyme-linked immunosorbent assay; and IL-33 gene polymorphisms were studied by realtime polymerase chain reaction.Results: IL-33 level was higher in patients with asthma (4.16±0.88pg/mL) compared to the control subjects (1.28±0.37 pg/mL) (p<0.001).The frequencies of AA, CC and AC alleles for the rs1342326 polymorphism were 52 (57.8%), 5 (5.6%) and 33 (36.7%) in the patient group, and 53 (58.9%), 7 (7.8%) and 30 (33.3%) in the control group, respectively.The frequencies of CC, TT and CT alleles for the rs1929992 polymorphism were 17 (18.9%),31 (34.4%), and 42 (46.7%) in the patient group and 13 (14.4%),41 (45.6%), and 36 (40%) in the control group, respectively.The frequencies of CC, TT and CT alleles for the rs3939286 polymorphism were 46 (51.1%), 7 (7.8%), and 37 (41.1%) in the patient group and 44 (48.9%), 9 (10%), and 37 (41.1%) in the control group, respectively.There was no statistically significant difference between the groups in terms of IL-33 gene polymorphisms (rs1342326, rs1929992 and rs3939286) (p=0.784,p=0.304, p=0.863, respectively). Conclusions:The current study showed increased serum IL-33 levels in patients with asthma compared to healthy controls but gene polymorphism studies did not show any significant difference.