Investigation of cytotoxicity and antibacterial effect of boron-containing nano-hydroxyapatite

OpenAlex İngilizce

Introduction: Different regenerative materials are used to protect and stimulate pulp tissue.Objectives: This in vitro study aimed to examine the cytotoxicity of different doses of boron-containing nanohydroxyapatite (B-nHAp) and its antibacterial effect.Material and methods: B-nHAp was synthesized with microwave-assisted chemical precipitation method at different rates, such as 1%, 5%, and 10%.The extracts were prepared in separate tubes: 1%, 5%, 10% B-nHAp, and nHAp 50 mg/ml.Cytotoxicity test was performed on L929 mouse fibroblasts for 24 and 72 hours.Antibacterial analysis was performed with Streptococcus mutans bacteria using micro-dilution method.Cell viability values were evaluated with two-way analysis of variance (ANOVA) test (p < 0.05).Results: B-nHAp was successfully synthesized in XRD and SEM analyses.Nano-hydroxyapatite showed more cell viability than control group at 24 and 72 hours.All B-nHAp extracts demonstrated less cell viability at the end of 24 hrs.than control group.However, 1% B-nHAp cell viability decreased more in the B-nHAp group compared with the control group, while 5% and 10% B-nHAp showed high cell viability after 72 hours of incubation.B-nHAp demonstrated less antibacterial effect on Streptococcus mutans compared with nHAp.Conclusions: According to the results, the addition of boron to nano-hydroxyapatite does not make a significant contribution to increase the regenerative and antibacterial properties of the material.