In Vitro Effect of Boron Compounds in Combination with Photobiomodulation Therapy by 905 nm on the Viability of Human Gingival Fibroblasts

OpenAlex İngilizce

This study aimed to evaluate the effect of Low-level laser therapy (LLLT) and Boric acid (BA) or Borax decahydrate (BD) on the cell survival of gingival fibroblasts.Fibroblast planted plates were divided into 10 groups: Control group, BD 100 µg/mL (BD 100), BD 200 µg/mL (BD200), BA 100 µg/mL (BA100), BA 200 µg/mL (BA200), LLLT, LLLT+BD100 µg/mL (LLLT+BD100), LLLT+BD200 µg/mL (LLLT+BD200), LLLT+BA100 µg/mL (LLLT+BA100) and LLLT+BA 200 µg/mL (LLLT+BA200) groups.LLLT was performed at a dose of 4 J/cm 2 for 160 sec.Following LLLT, MTT analysis was performed after the cells were kept in the incubator for 24 h.The LLLT+BA 200 group exhibited the highest cell density.In MTT analysis, significantly higher cell numbers were observed in the BA200, LLLT+BA100, and LLLT+BA200 groups compared to the control group (P<0.05).Then, the 3-(4,5-dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide assay and 8-hydroxy-2'-deoxyguanosine and Bcl-2 Associated X-Protein were analyzed.The 8-hydroxy-2'-deoxyguanosine and Bcl-2 Associated X-Protein levels were decreased in the LLLT+BA200 group compared to the control group (P<0.05).This study suggests that BA200, LLLT+BA100, and LLLT+BA200 increase the survival of fibroblast cells.