Dentin tubule penetration of a calcium silicate–based sealer after removal of calcium hydroxide or N-acetylcysteine medicaments: an in vitro study

OpenAlex İngilizce

BACKGROUND: ) or N-acetylcysteine (NAC) intracanal medicaments with needle irrigation or EDDY sonic activation. METHODS: (Kalsin) or (NAC) for 7 days. Medicaments were removed using conventional needle irrigation or EDDY sonic activation. Specimens were obturated with a calcium silicate-based sealer Bioserra using the single-cone technique. CLSM quantified residual medicament and sealer penetration into dentin tubules. Statistical analyses were performed using the Mann-Whitney U and Kruskal-Wallis tests, with the significance level set at p < 0.05. RESULTS: Neither the type of intracanal medicament nor the activation technique significantly affected the residual medicament amount (p > 0.05). However, the type of medicament significantly influenced sealer penetration, with NAC showing greater dentin tubule penetration than Ca(OH)₂ (p < 0.05). The activation technique did not significantly influence sealer penetration (p > 0.05). Sealer penetration was highest in the coronal third and lowest in the apical third (p < 0.05). CONCLUSION: Within the limitations of this in vitro study, NAC enhanced the dentin tubule penetration of a calcium silicate-based sealer compared with Ca(OH)₂. The activation technique did not affect sealer penetration or residual medicament.