Protective effect of exendin-4 treatment on oxidative status of liver in rats exposed to chronic methylglyoxal

OpenAlex İngilizce

The protective effects of Glucagon-Like Peptide-1 (GLP-1) agonists against oxidative stress-induced cellular injury have been well established by previous experimental and clinical studies.Male Wistar rats (200-250 g weight, n=24) were used in this study.First group of rats were not treated with Methylglyoxal (MGO) and served as control group (C group).Second group of rats (MGO group) received MGO (75 mg/ kg/day in drinking water) for 12 weeks.Third group of rats (MGO+Ex-4) received Exendin-4 (Ex-4) (1 μg/kg twice daily subcutaneously) concomitant with MGO for 12 weeks.At the end of the 12th week, total oxidant status (TOS), total antioxidant capacity (TAC), sulfhydryl groups (SH), myeloperoxidase (MPO), and advanced oxidation protein products (AOPP) in the liver tissues of all groups were measured spectrophotometrically.In MGO-administered rats, TOS, MPO and AOPP levels were significantly increased.Treatment with Ex-4 for 12 weeks caused a significant decline in the levels of these markers in rats exposed to MGO.Also, levels of TAC and SH were decreased significantly after the 12 weeks of MGO administration.