Hemimycena lactea on Pine Cones: First Molecular Confirmation in Türkiye

Türkçe

In this study, a specimen of Hemimycena lactea (Acar 2041) was examined using morphological and molecular data. The specimen was collected on a pine cone from Çanakkale (Yapıldık Village, Derinkoy area) on January 28, 2025. The species is consistent with its description in the literature, with its completely white and delicate basidiomas, translucent-striate pileus margin, sparse lamellae, and thin stipe. Microscopically, the spores were found to be ellipsoidal–subfusiform in shape, and the spore wall was found to be inamyloid (Q ≈ 2). Microscopically, basidia with 2–4 sterigmata, clamp connections, and diverticulate-like pileipellis elements were observed; the spores were ellipsoidal–subfusiform and inamyloid (Q ≈ 2). In the context of Bayesian phylogenetic analysis, based on the ITS rDNA sequence (29 sequences; 27 in-groups, 2 out-groups), the sequence obtained in this study (GenBank: PZ091910) was placed within a highly supported (PP = 1.00) monophyletic clade with reference H. lactea sequences. The molecular results obtained from this study serve to confirm the species identification, and the observation of its growth on conifer cones during the winter period provides novel data on the ecological tolerance of the species.