House dust mite specific in vitro IgE determination in cats with allergic dermatitis

İngilizce (OpenAlex)

Allergy, nearly a hundred years ago, a paediatrician comprehended the idea of allergy, the initial connection of the immune system via an antigen altering the reactivity of the cases. In recent years several hypothesis and novel findings have been conducted in veterinary medicine, yet allergic dermatitis due to house dust mites ar of interest in feline medicine. The purpose of this retrospective reserach was to verify underlying causes of clinical findings to those of cats with allergic dermatitis. Allergen speicific Ig E concentrations against Dermatophagoides farinae and Dermatophagoides pteronyssinus were determined by use of in vitro Polycheck Veterinary allergy tests (RDA grup, İstanbul). In a total of 33 cats were introduced to the Adnan Menderes University, Faculty of Veterinary, Department of Internal Medicine clinic with primary/secondary skin lesions or to those of general health status check up. On initial referaal available haematological, microbiological, parasitological and relevant tests were performed. Nineteen cats were deemed healthy based on relevant analysis (neither infection nor hypersensitivity) were enrolled as a control group. Other 14 cats were composed of allergic cases. A total 0.2 ml of blood was taken from all cats for specific IgE analysis against 20 different allergens, in which interpretation involved solely house dust mites. Regarding allergen specific IgE levels (kU/l) in allergic cats, in vitro tests gave a positive reactions for D. farinae [class 3,4 (n=1), class 2 (n=7), class 1 (n=6) and class 0 (n=0)] and D. pteronyssinus [class 3,4 (n=1), class 2(n=5), class 1(n=4) and class 0 (n=4)] in whom at least 1 antigen was detected in all allergic cats. There was a statistical significance for specific Ig E leves between healthy controls and allergic cats against house dust mites as follows; 0.21 ± 0.018 vs. 4.69 ± 1.49 for D. farinae (p=0.0001) and 0.21 ± 0.023 vs. 3.11 ± 1.21 (p=0.003) for D. pteronyssinus. In the present retrospective study, all positive reactions on the Polycheck allergy test were suggested to present underlying house dust allergens of dermatitis in enrolled cats. As far as well known factor that in vitro allergy tests solely might not be reflecting hypersensitivity from healthy cases because of nonunique clinically findings