BIOCHEMICAL AND DNA DAMAGE RESPONSES OF HYDROPONICALLY GROWN ELANDS SOURFIG (CARPOBROTUS ACINACIFORMIS L.) LEAVES TO CADMIUM STRESS CONDITIONS

OpenAlex İngilizce

Biochemical responses, DNA damages, and cadmium (Cd) accumulation capacity of Elands sourfig (Carpobrotus acinaciformis L.), a halophyte plant, were investigated under a variety of CdNO3 concentrations in hydroponic conditions for 30 days.Total chlorophyll/carotenoid and proline contents of the plants under differing Cd concentrations (0-, 2.5-, 5-, 10-, 20-, 40-, 60-, 80 and 100 ppm) gradually increased up to 20 and 40 ppm, respectively, which was followed by a decrease, P≤0.05.Accumulation of malondialdehyde (MDA, 3.22 to 22.87 nmol g -1 fresh weight, Fwt) and H2O2 (2.93-24.14µmol g -1 Fwt) contents showed an increasing trend along with higher Cd concentrations, P≤0.05.On the other hand, antioxidant enzymes such as peroxidase (POX) and catalase (CAT) exhibited increasing trends only up to 40 ppm of Cd concentrations and the following drastic decline was evident, P≤0.05.The content of Cd levels in those leaves increased from 0.01 to 65.77 mg kg -1 with respect to Cd concentrations.DNA in leaves was not fragmented up to 5 ppm Cd level.When DNA damage was assayed in a single cell via comet assay, 80-and 100 ppm of Cd toxicity resulted in DNA breaks.Measuring DNA damage via comet assay could be a very useful approach to find out what level of Cd toxicity could be tolerated if a particular halophyte plant is used to remediate the Cd-contaminated media.