Detection of Ibuprofen Levels by High Performance Liquid Chromatography After Phonophoresis in the Tissues of Patients with Knee Osteoarthritis: A Controlled Preliminary Study

OpenAlex İngilizce

ABS TRACT Objective: This study is aimed to detect the drug concentration in articular tissues after phonophoresis application in patients with knee osteoarthritis who were planned to undergo total knee arthroplasty.Material and Methods: Seventeen patients with grade 4 knee OA were allocated to 6 groups according to ultrasound parameters and three sessions of ibuprofen phonophoresis were applied before surgery.Six groups were divided into as follows: Group 1; 5 minutes, frequency 0 MHz, power 0 W/cm 2 , continuous mode; Group 2; 5 minutes, frequency 1 MHz, power 1 W/cm 2 , continuous mode; Group 3; 5 minutes, frequency 1 MHz, power 1.5 W/cm 2 , continuous mode; Group 4; 5 minutes, frequency 1 MHz, power 1.5 W/cm 2 , pulse mode; Group 5; 8 minutes, frequency 1 MHz, power 1.5 W/cm 2 , continuous mode; Group 6; 5 minutes, frequency 1 MHz, power 2 W/cm 2 , continuous mode.Bone, synovial fluid and synovial tissue samples were obtained from patients during the surgery.A high-performance liquid chromatographic method was used for the determination of ibuprofen levels from isolated human synovial fluid, synovial tissue and bone.Results: Ibuprofen was detected to the articular tissues of patients.The highest concentrations of ibuprofen in synovial fluid were detected in Group 3 and 5.The highest concentrations in bone were obtained in Group 2 and in synovial tissue were obtained in Group 2 and Group 3. Conclusion: Optimal penetration of ibuprofen to articular tissues was obtained with 1 MHz and 1-1.5 W/cm 2 , and continuous ultrasound mode.Phonophoresis is seemed as an effective treatment modality in patients with OA.