Influences of Light-Emitting Diodes (LEDs) and Culture Media on Micropropagation of Native Hemp (Cannabis sativa) Population from Türkiye

OpenAlex İngilizce

Light-emitting diodes (LEDs) have historically been the standard for supplemental lighting in industrial hemp (Cannabis sativa) cultivation in a greenhouse, but there is a lack of scientific understanding in C. sativa regarding different media under various LEDs in vitro conditions. The aim of this study was to determine the top-performing LEDs and nutrient media for micropropagation of C. sativa. In this study, two different spectra (white and red LEDs) and different media [MS, B5, MS+B5, ½ MS, ½ B5, ½ MS + ½ B5, and control (without medium)] were used, maintaining constant light photoperiod. The diversity of light spectra and nutrient media showed statistically significant differences for the growth parameters of C. sativa. Red LED provided an extraordinarily high shoot length of 7.37 cm, whereas white LED exhibited the highest root length with 6.57 cm. Also, nutrient media were found to be effective on shoot growth in full-strength media, while on root growth in half-strength media. Among the plant growth parameters, the number of shoots and nodes per plant is of great importance for clonal propagation. B5 medium under red LED provided an extraordinarily high shoot number with 4.64, whereas MS medium under white LED exhibited the highest node number with 5.87. Each LED source required the respective medium adjustment for the best results of the desired parameters. In this respect, the study may be advantageous for the selection of the appropriate nutrient medium and led spectrum in micropropagation of C. sativa.