Makale detayı · 2022
DETERMINATION OF THE EFFECTIVENESS OF SILDENAFIL ON HUMAN AORTIC SMOOTH MUSCLE CELL
Journal of Applied Biological Sciences
- Yıl
- 2022
- ISSN
2146-0108- Tür
- article
Veri kaynağı ayrımı
- YÖKSİS YÖKSİS makale kaydı
- OpenAlex OpenAlex zenginleştirmesi (özet, atıf, konular)
Özet
İngilizce (OpenAlex)
Erectile dysfunction is defined as the inability to gain and/or maintain a penile erection sufficient for satisfactory sexual performance. Sildenafil is a phosphodiesterase type 5 (PDE5) inhibitor used in the treatment of erectile dysfunction. The aim of this study is to investigate the effect of sildenafil (0.8% in cell media) on the mechanism of human aortic smooth muscle contraction. Human smooth muscle cells treated with sildenafil for 1 and 4 days. These cells were then homogenized, and enzymes and proteins responsible for the mechanism of intracellular contraction were analysed. Rho-kinase (ROCK) activity, RhoA, ROCK II, CPI-17α, PDE5a, and PLC levels were evaluated by using ELISA. In this study, it was observed that sildenafil increased RhoA, PDE5a and CPI-17α levels, and decreased ROCK enzyme activity.
Konular
- Extracellular vesicles in disease
- Osteoarthritis Treatment and Mechanisms
Birincil konu Extracellular vesicles in disease