Investigation of the Protective Effect of Nigella Sativa Oil in Cisplatin Induced Oral Mucositis: An Experimental Study

OpenAlex İngilizce

Objective: The aim of this experimental study was to investigate the histopathologic effect of Nigella Sativa oil (NSO) on cisplatin (Cis) induced oral mucositis (OM) in rats.Methods: Twenty-four rats were divided into four equal groups.The animals in Group 1 and Group 2 were given 5 mg/kg intraperitoneal (ip) Cis systemically on the 1 st , 3 rd and 5 th days of the study.Additionally, 15 mL NSO were given to the rats in Group 2, with gavage feeding on days 1, 3, 5, 7, and 9.The animals in Group 3 were given per oral 15 ml NSO on days 1, 3, 5, 7 and 9.As the control group, Group 4 received a total of 15 mL 0.9% saline solution divided into 5 doses on days 1, 3, 5, 7 and 9 by oral gavage.On the 14 th day, animals were euthanized and buccal mucosa from both sides, including submucosal tissues, were excised and taken to histopathological examination.Results: The mean mucosal thicknesses of the groups were 224.58 µm, 276.1 µm, 323.33 µm, and 331.33 µm, respectively for Groups 1, 2, 3, and 4 (p<0.05).When the degree of mucosal inflammation was examined, the most intense inflammation was detected in Group 1 and the least intense inflammation was in Group 4 (p<0.01).The degree of inflammation in Group 2 and Group 3 were similar to Group 4 (p>0.05).Conclusion: According to the results of this study, NSO, for which anti-inflammatory and antioxidant properties have been shown in previous studies, may also be effective in preventing Cis-induced OM.