Highly Sensitive and Disposable DNA‐Based Electrochemical Biosensor for Detection of Daunorubicin

İngilizce (OpenAlex)

Despite the indisputable efficacy of chemotherapy in the treatment of cancer, the adjustment of chemotherapy drug dosage remains a concern. Therefore, herein, a flower‐like nanomaterial is developed as an electrochemical nanobiosensor for cancer drug determination, considering the advantages of providing a lower limit of detection (LOD) by increasing the electrode surface area. A flower‐like porous manganese oxide (MnO2) is synthesized and modified onto the surface of a pencil graphite electrode (PGE). It is used to investigate the interaction between dsDNA and daunorubicin (DNR), as an anticancer drug, using the voltammetric technique. As a result of the measurements made with developed MnO2‐PGE using DPV, the LODs of dsDNA and DNR are calculated as 0.62 μg mL−1 and 54 nM, respectively. The dsDNA–DNR interaction investigation revealed that, in just three minutes, the maximum interaction is achieved and a 26.9% reduction in the dsDNA guanine signal is noted. On the other hand, there is an 81.2% decrease in the DNR oxidation signal. Kinetic investigation shows a binding constant (K) of 1.81 × 106 M−1 between dsDNA and DNR. The LOD of DNR using the dsDNA/MnO2‐PGE surface is calculated as 0.06 μM in a linear dynamic range of 0.1–0.5 μM.