Yabani Çuhaçiçeğinin Doku Kültürü İle Kitlesel Üretimi

OpenAlex İngilizce

Primrose (Primula spp.), in the Primulaceae family, blooms in early spring and is produced as a seasonal flower or potted ornamental. Even though primroses can be propagated by separating the offspring from the mother plant, the reproduction coefficient is low and the irregular and late germination seen in their seeds that prevent mass production. Therefore, with this study, it was aimed to mass production of wild primroses, which spread naturally, by culturing in vitro. Plants collected from nature were cultured in greenhouse conditions and used as explant source. Surface sterilized rhizome explants were cultured in MS medium containing growth regulators Benzyl adenine (BA) (0.5, 1.0 and 3.0 mg L-1) and Indole butyric acid (IAA) (0.5 mg L-1). As a result of the study, the highest multiplication coefficient was 7.85 in MS medium containing 1.0 mg L-1 BA and 0.5 mg L-1 IAA. Rooting success of 75% was achieved by culturing the shoots in MS medium containing 1 mg L-1 IBA. A survival rate of 62.5% was obtained in plants cultured in peat:perlite medium.