Genotype-specific responses to in vitro drought stress in myrtle (Myrtus communis L.): integrating machine learning techniques

OpenAlex İngilizce

Background: L.), native to the Mediterranean region of Türkiye, is a valuable plant with applications in traditional medicine, pharmaceuticals, and culinary practices. Understanding how myrtle responds to water stress is essential for sustainable cultivation as climate change exacerbates drought conditions. Methods: drought stress by employing tissue culture techniques, rooting trials, and acclimatization processes. Genotypes were tested under varying polyethylene glycol (PEG) concentrations (1%, 2%, 4%, and 6%). Machine learning (ML) algorithms, including Gaussian process (GP), support vector machine (SVM), Random Forest (RF), and Extreme Gradient Boosting (XGBoost), were utilized to model and predict micropropagation and rooting efficiency. Results: The research revealed a genotype-dependent response to drought stress. Black-fruited genotypes exhibited higher micropropagation rates compared to white-fruited ones under stress conditions. The application of ML models successfully predicted micropropagation and rooting efficiency, providing insights into genotype performance. Conclusions: The findings suggest that selecting drought-tolerant genotypes is crucial for enhancing myrtle cultivation. The results underscore the importance of genotype selection and optimization of cultivation practices to address climate change impacts. Future research should explore the molecular mechanisms of stress responses to refine breeding strategies and improve resilience in myrtle and similar economically important crops.