Phytochemical Study and Antioxidant Activities of the Water-Soluble Aerial Parts and Isolated Compounds ofThymus munbyanus subsp. ciliatus (Desf.) Greuter & Burdet

OpenAlex İngilizce

Objectives: The present study aimed to determine the phenolic compounds present in the water-soluble extracts of Thymus munbyanus subsp.ciliatus using high pressure liquid chromatography-time-of-flight mass spectrometry (MS).These phenolic compounds were further isolated and characterized for their antioxidant activities. Materials and Methods:The aerial parts of T. munbyanus subsp.ciliatus were air dried, powdered, and extracted using water:methanol three times.The concentrated hydromethanolic extract was further dissolved in H 2 O, filtered, and successively extracted using ethyl acetate, chloroform, and n-butanol.T. munbyanus extracts were further purified using column chromatography, and the purified extracts were subjected to in vitro antioxidant assays.Results: Two previously undescribed compounds, namely methyl 2,3,5,6-tetrahydroxybenzoate and 4-hydroxy-5-methoxy-2-oxo-2H-pyran-3-carboxylic acid, and 14 known compounds, including 3 flavonoids; namely 3',5,5',7-tetrahydroxyflavanone, luteolin, and isorhamnetin-3-O-βglucoside; a sterol glucoside named daucosterol; and 10 phenolic compounds, namely salicylic acid, ferulic acid, pluchoic acid, ethyl caffeate, methyl caffeate, protocatechuic acid, rosmarinic acid, p-coumaric acid, tyrosol, and protocatechuic aldehyde, were isolated from ethyl acetate and n-butanol extracts.The isolated compounds were characterized using 1D-2D -1 H -13 C nuclear magnetic resonance and MS methods.Conclusion: The compounds isolated from ethyl acetate and n-butanol extracts exhibited excellent antioxidant and 2,2-diphenyl-1-picrylhydrazyl scavenging activities.All these results highlighted the antioxidant potential of the isolated phenolic compounds and extracts, which could be further utilized for different pharmacological applications.