Identification of virulence resistance genes in Pseudomonas aeruginosa strains isolated from blood samples

OpenAlex İngilizce

Aim: In this it is aimed to the determine the presence of virulence resistance genes (toxA, algD, plcN, lasB, plcH) in P. aeruginosa isolates isolated from blood samples. Materials and methods: DNA extraction of the study isolates was done by boiling method. Optimization was done using positive control after DNA extraction. After optimization, the presence of virulence (toxA, algD, plcN, lasB, plcH) resistance genes was investigated by polymerase chain reaction (PCR) method. Results: As a result of PCR of the virulence gene regions (toxA, algD, lasB, plcN, plcH); Positive rates of KR 25 isolates were 80% (n=20), 100% (n=25), 100% (n=25), 100% (n=25), 96% (n=24) in KS 46 isolates. On the other hand, the positive rate distributions were found to be 93.47% (n=43), 100% (n=46), 100% (n=46), 100% (n=46), 97.82% (n=45). Conclusion: As a result of PCR of virulence gene regions (toxA, algD, lasB, plcN, plcH) of P. aeruginosa, it was determined that algD, lasB, plcN genes were found in all 25 carbapenem resistant (KR) and 46 carbapenem sensitive (KS) isolates.