Evaluation of Antioxidant Potential, Anticholinesterase Activity and Element Content of Chlorophyllum rhacodes Species

OpenAlex İngilizce

In this study, antioxidant potential, anticholinesterase activity and elemental contents of Chlorophyllum rhacodes were evaluated. In order to determine the antioxidant capacity, total antioxidant level (TAS), total oxidant level (TOS), oxidative stress index (OSI), DPPH radical scavenging activity and FRAP ferric reducing power analyses were applied. According to the obtained results, TAS value was determined as 3.125±0.060 mmol/L, TOS value as 6.821±0.062 µmol/L, OSI value as 0.218±0.003, DPPH activity as 55.430±1.438 mg TE/g and FRAP value as 72.540±1.461 mg TE/g. These data revealed that C. rhacodes showed a significant level of antioxidant activity. In the evaluation of anticholinesterase activity, inhibitory effects against AChE and BChE enzymes were determined and IC₅₀ values ​​were determined as 94.330±1.536 µg/mL and 128.397±1.556 µg/mL, respectively. These results show that C. rhacodes shows limited inhibitory effect on these enzymes but may have a certain level of anticholinesterase potential. According to the results of elemental analysis, Cd (14.78±1.53), Cr (33.17±1.42), Mn (7.35±0.52), Cu (155.77±2.34), Fe (469.30±15.75), Pb (6.98±0.54), Ni (3.66±0.31) and Zn (76.18±2.25) mg/kg levels were detected in mushroom samples. In particular, it was observed that Cd and Cu levels were above the upper limits specified in the literature. This situation shows that C. rhacodes tends to accumulate some heavy metals and may be highly sensitive to environmental pollution. In conclusion, C. rhacodes species exhibits a biologically remarkable profile with certain antioxidant and anticholinesterase activities and can also be considered as a potential indicator species in the biological monitoring of environmental metal exposure.