Makale detayı · 2009
Extraction and Inhibition of Enzymatic Activity of Botulinum Neurotoxins A1 A2 and A3 by a Panel of Monoclonal Anti BoNT A Antibodies
- Yıl
- 2009
- Tür
- article
Veri kaynağı ayrımı
- YÖKSİS YÖKSİS makale kaydı
- YÖKSİS dergi adı PLoS ONE
- Katalog eşleşmesi (ISSN) PLOS ONE
- OpenAlex OpenAlex zenginleştirmesi (özet, atıf, konular)
Özet
OpenAlex · İngilizce
Botulinum neurotoxins (BoNTs) are extremely potent toxins that are capable of causing death or respiratory failure leading to long-term intensive care. Treatment includes serotype-specific antitoxins, which must be administered early in the course of the intoxication. Rapidly determining human exposure to BoNT is an important public health goal. In previous work, our laboratory focused on developing Endopep-MS, a mass spectrometry-based endopeptidase method for detecting and differentiating BoNT/A-G serotypes in buffer and BoNT/A, /B, /E, and /F in clinical samples. We have previously reported the effectiveness of antibody-capture to purify and concentrate BoNTs from complex matrices, such as clinical samples. Because some antibodies inhibit or neutralize the activity of BoNT, the choice of antibody with which to extract the toxin is critical. In this work, we evaluated a panel of 16 anti-BoNT/A monoclonal antibodies (mAbs) for their ability to inhibit the in vitro activity of BoNT/A1, /A2, and /A3 complex as well as the recombinant LC of A1. We also evaluated the same antibody panel for the ability to extract BoNT/A1, /A2, and /A3. Among the mAbs, there were significant differences in extraction efficiency, ability to extract BoNT/A subtypes, and inhibitory effect on BoNT catalytic activity. The mAbs binding the C-terminal portion of the BoNT/A heavy chain had optimal properties for use in the Endopep-MS assay.
Konular
Atıflar
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67 atıf
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Yerel katalogda bu makaleye atıf yapan 6 yayın (OpenAlex referans eşleşmesi; tam dünya listesi değildir).
- Neutralizing human monoclonal antibodies binding multiple serotypes of botulinum neurotoxin 2011
- Neutralizing human monoclonal antibodies binding multiple serotypes of botulinum neurotoxin 2011
- Affinity maturation of human botulinum neurotoxin antibodies by light chain shuffling via yeast mating 2010
- Affinity maturation of human botulinum neurotoxin antibodies by light chain shuffling via yeast mating 2010
- Extraction and inhibition of enzymatic activity of botulinum neurotoxins /B1, /B2, /B3, /B4, and /B5 by a panel of monoclonal anti-BoNT/B antibodies 2011
- Extraction and inhibition of enzymatic activity of botulinum neurotoxins/B1, /B2, /B3, /B4, and /B5 by a panel of monoclonal anti-BoNT/B antibodies 2011