Makale detayı · 2021
l-asparaginase immobilized p(HEMA-GMA) cryogels: A recent study for biochemical, thermodynamic and kinetic parameters
- Yıl
- 2021
- Tür
- article
Veri kaynağı ayrımı
- YÖKSİS YÖKSİS makale kaydı
- YÖKSİS dergi adı Polymer Testing
- Katalog eşleşmesi (ISSN) Polymer Testing
- OpenAlex OpenAlex zenginleştirmesi (özet, atıf, konular)
Özet
OpenAlex · İngilizce
Cryogels have recently been attracted intense attention as suitable carriers for enzyme immobilization. Herein, l-asparaginase was selected as the model enzyme due to its application such as pharmaceutical and food. Under optimum conditions, l-asparaginase was immobilized on poly (2-hydroxyethyl methacrylate-glycidyl methacrylate) cryogels with 68.8% of immobilization yield and 69.3% of activity recovery. The immobilized enzyme exhibited improved stability with respect to the soluble enzyme at extreme conditions, especially around acidic pH and high temperature. Also, the storage stability and reusability of the immobilized enzyme were found to be approximately 54% and 52% of the original activity after 28 days at room temperature and 10 cycles, respectively. The thermodynamic studies indicated that activation energy (Ea) of the free enzyme decreased from 13.08 to 10.97 kJ/mol, which means an increase in the thermostability of l-asparaginase. The Michaelis-Menten constants (Km) of 2.04 and 1.67 mM, and the maximum reaction rates (Vmax) of 170.0 and 115.0 μM min−1 were estimated for soluble and immobilized l-asparaginase, respectively. These findings demonstrated that the designed cryogels turn out to be a good carrier matrix for l-asparaginase immobilization with high catalytic efficiency and enhanced stability.
Konular
Atıflar
OpenAlex cited_by_count. WoS veya Scopus atıf sayısı değildir; o kaynaklar için ayrı kolon yoktur.
44 atıf
OpenAlex cited_by_count (önbellek / veritabanı)
Yerel katalogda bu makaleye atıf yapan 25 yayın (OpenAlex referans eşleşmesi; tam dünya listesi değildir).
- Immobilization of Xylanase into Zeolitic Imidazolate Framework-67 (ZIF-67) and Manganese-Doped ZIF-67 (Mn/ZIF-67): A Comparison Study 2024
- Immobilization of collagenase in inorganic hybrid nanoflowers with enhanced stability, proteolytic activity, and their anti-amyloid potential 2024
- Newly Synthesized Multifunctional Biopolymer Coated Magnetic Core/Shell Fe3O4@Au Nanoparticles for Evaluation of L-asparaginase Immobilization 2023
- Newly Synthesized Multifunctional Biopolymer Coated Magnetic Core/Shell Fe3O4@Au Nanoparticles for Evaluation of L-asparaginase Immobilization 2023
- Newly Synthesized Multifunctional Biopolymer Coated Magnetic Core/Shell Fe3O4@Au Nanoparticles for Evaluation of L-asparaginase Immobilization 2023
- Inhibition of bacterial adhesion by epigallocatechin gallate attached polymeric membranes 2023
- Newly Synthesized Multifunctional Biopolymer Coated Magnetic Core/Shell Fe3O4@Au Nanoparticles for Evaluation of L-asparaginase Immobilization 2023
- Inhibition of bacterial adhesion by epigallocatechin gallate attached polymeric membranes 2023
- A Positive Effect of Magnetic Field on the Catalytic Activity of Immobilized L-Asparaginase: Evaluation of its Feasibility 2023
- A new design to enhance the enzyme activities: Investigation of L-asparaginase catalytic performance by IMAC effect on g-C₃N₄ nanolayers 2026