The Effect of Tarantula Cubensis Alcoholic Extract on Advanced Oxidation Protein Products and Total Sulfhydryl Levels in The Stomach Tissue of Gastric Damaged Rats with Indomethacin

İngilizce (OpenAlex)

There are many markers used to assess the oxidative stress state of the cell. Advanced oxidative protein products (AOPP), which are formed by the reaction of oxygen radicals with proteins in the cell, are one of these markers. Total sulfhydryl (t-SH) is accepted as an indicator of antioxidant status. Alcoholic extract of the spider Tarantula Cubensis (TCAE) is used as a homeopathic agent in the Veterinary medicine. TCAE has many therapeutic effects such as anti-inflammatory, antitumor, necrotizing action and wound healing. In this study, it was aimed to investigate the effect of TCAE on AOPP and t-SH levels in the stomach tissue of rats with indomethacin-induced gastric damage. For this purpose, the rats were divided into 4 groups (n=7) as control, Indomethacin (25 mg/kg indomethacin), Indomethacin + Lansoprazole (25 mg/kg indomethacin +30 mg/kg lansoprazole) and Indomethacin +TCAE (25 mg/kg indomethacin + 200 µg/kg TCAE) group. Five minutes after lansoprazole and TCAE administration, all rats except the control group were given a single dose of indomethacin (25 mg/kg) orally, and six hours after indomethacin administration, the gastric tissue was removed for biochemical analysis under anesthesia. Spectrophotometric method was used to measure AOPP and t-SH levels in gastric tissue. In the study, the effects of TCAE were compared with the effects of lansoprazole used in the treatment of ulcers. A statistically significant (p<0.05) increase was found in the AOPP and t-SH levels in the indomethacin group compared to the control group. AOPP and t-SH levels were found to be lower in the Indomethacin + TCAE group compared to the Indomethacin and Indomethacin + Lansoprazole groups. According to the results obtained in this study, it was determined that TCAE could prevent oxidative damage in gastric tissue by inhibiting the increase in AOPP and tSH levels caused by indomethacin administration, and this effect was stronger than lansoprazole.