A meta-analysis of the overexpression of AP2/ERF genes in response to drought stress in plants

İngilizce (OpenAlex)

The APETALA2/ethylene-responsive element-binding factor (AP2/ERF) family is primarily known for regulating plant development; however, it also participates in abiotic stress responses. Ethylene response factor (ERF) and dehydration responsive element-binding (DREB) transcription factors (TFs), the subgroups of this superfamily, are especially active in drought response. A thorough meta-analysis has yet to be performed despite the numerous studies conducted on the overexpression of the AP2/ERF superfamily in various plant species. In the present study, a comprehensive meta-analysis was performed to investigate the effects of AP2/ERF overexpression on drought stress. The analysis was conducted using 35 studies on 20 different species. A total of ten moderator analyses were performed, and summary effect analysis demonstrated that AP2/ERF members, namely ERF and DREB overexpression, notably enhanced the survival rate (P = 0.0275) and proline content (P = 0.0000) under drought conditions. Overexpression also resulted in elevated levels of catalase and peroxidase activity, hydrogen peroxide, photosynthesis rate, plant dry weight, plant fresh weight, root length, shoot dry weight, stomatal conductance, and transpiration rate under drought conditions compared to the control group; however, a negative effect on malondialdehyde level, relative water content, ion leakage, and singlet oxygen. The primary conclusion of this meta-analysis research is that AP2/ERF overexpression leads to an increase in proline levels and a rise in the survival rate of plants under drought stress. The results of this study are expected to provide valuable insights for future research in the AP2/ERF superfamily